Fuli’s Mark of the Guard is usually replaced by a circular ring mark and or she is missing the circular ring mark she has on her left shoulder.
Beshte and other hippos have three toes on each hoof, while real hippos have four toes on each hoof.
Fuli has retractable claws, while real cheetahs have non-retractable claws that act like spike shoes.
Makucha, Badili, and Mapigano have non-retractable claws, while real leopards have retractable claws, and only extend their claws when they need them.
Despite being a juvenile, Ono has orange breeding plumes, which in reality are only present on adult egrets.
Janja the hyena is the leader of his clan, despite being a male. In reality, female hyenas are dominant.
Hyenas are portrayed yelping like dogs, but real hyenas are not dogs nor do they sound like them.
Kiara the lioness is destined to become the leader of her pride since she is the oldest child of Simba and Nala, despite being a female. In reality, male lions are dominant.
Zira is also the leader of the Outsiders, despite being a female.
Female ostriches in the show such as Mbuni have black feathers, despite the fact that only males have black feathers in real life. Females have brown plumage to help them blend in with the nest.
Kambuni bears the coloring of an adult male ostrich rather than a female chick.
Fuli lacks the streaks under her eyes that all real cheetahs have.
Although he's identified as an Egyptian cobra, Ushari bears the external hood markings of an Indian cobra- a species that does not occur in Africa.
Zebras make a horse neigh sound, but zebras do not neigh like horses; they bark like jackals or bray like donkeys.
Only Makuu's teeth on his top jaw stick out when his mouth is closed like on an alligator, despite him being a crocodile.
Snakes are shown blinking or closing their eyes throughout the series, despite the fact that snakes lack eyelids.
The tongues of giraffes in the series are pink rather than the blue-black hue seen on real giraffes.
Swala and other female gazelles horns are the same sizes as the male gazelles, despite them being drastically shorter than the males' horns in real life.
Muhimu's son Hamu got up and walked right after he was born. In real life, it takes about fifteen minutes after birth to do this. In addition to this, a few minutes after he is born, he kicks Janja, but it takes real zebras about two days after birth to run and kick properly.
Jasiri's quote, "We're the clean-up crews" and Ono's quote "hyenas are scavengers", are not completely accurate. Ninety percent of a spotted hyena's diet is food they've caught themselves, so only ten percent of their food consists of leftovers. The only hyena species that actually are scavengers are the striped hyena and the brown hyena.
The rosette shape of Fuli's spots indicates that she is a mutation of cheetah called the king cheetah, whose spots are darker and more abstract (though they do not have rosettes, Fuli's spots resemble a simplified version of a king cheetah's markings). However, king cheetahs have three black stripes down their back, which Fuli does not have.
There is a painting of an elk in The Lair of the Lion Guard. However, elks are native to North America and Asia, and cannot be found in Africa.
Ushari is shown restraining Bunga by constricting, which cobras are incapable of in real life.
During the Trail of Udugu, Kion and Kiara find paw prints on the ground and assume it's Nala's prints. However, because the claw marks were sticking out, they're probably dog prints.
There is a mention of reindeer in Timon and Pumbaa's Christmas. However, reindeer are native to North America, Europe and Asia, and cannot be found in Africa.
Bunga is blue and white, but real life honey badgers are black and white.
The monkeys are shown to have prehensile tails that can grab onto tree branches. This physical feature is only available in New World monkeys of Central and South America.
Despite being both female and a juvenile, Makini has the vibrant markings of an adult male mandrill.
Makuu and the crocodiles not only are able to get close to Basi's Pod, who peacefully allow them to, but are also able to force the hippos out of the Big Springs. In real life, hippos are one of the most aggressive animal species in the world, and are known to have the ability to bite an adult crocodile in half, as well as being highly territorial towards anything that crosses their path.
Dhahabu is a full-grown golden zebra. In the real world, they wouldn't survive up to adulthood unless they are in captivity.
As a young cub, Fuli didn't have grey fuzzy hair on her head and back as real cheetah cubs do.
Kinyonga the chameleon can change her colors really fast, but that can't be done with real chameleons. Chameleons can´t turn invisible like Kinyonga can.
Kinyonga appears to be a veiled chameleon, but that species lives in the Arabian Peninsula rather than Africa.
Mtoto's tusk seem to be growing out of his lower jaw, but in real life elephants tusk grow out of the upper jaw.
Ma Tembo's herd includes bull elephants, but in real life bulls live separate from herds.
Bees are depicted living inside hives that resemble hornet nests. Real beehives are just honeycombs dangling from trees or lining the inside of hollow trees.
Impalas are all depicted having horns, which only males possess.
Tamaa is supposedly a fork-tailed drongo, but he has tail streamers and a head crest like a greater racket-tailed drongo- which does not occur in Africa.
Mpishi is referred to as a hawk by the characters including herself, but harrier hawks are not true hawks at all.
Aardwolves are stated to look like hyenas and take offense when being called one. Aardwolves actually are a type of hyena.
Kifaru and Mwenzi's friendship perpetrates the outdated belief that oxpeckers have a symbiotic relationship with rhinoceroses. In real life, oxpeckers are actually parasites feeding on the blood of larger animals, even pecking wounds to get it.