Ma Tembo is seen at her father's funeral with the rest of her herd. She watches the other elephants lay their tributes, and smiles when a baby elephant lays their flowers down upon the corpse of her late father.
Later, she announces that Simba will be saying a few words about her father. She listens to Simba's mistake in speaking elephantese, and, although at first appears surprised, she simply laughs. She admits that her father did indeed have 'poop' on him, and then thanks Simba, whilst recalling amusing times with her father.
Ma Tembo hosts The Elephants' Concert, although Simba is unable to attend after falling down a sink hole. She is seen conducting the concert, with Kiara and Nala attending instead. She is honored to have two members of the royal family attend, and she shows her respect by bowing to them. Shortly after the concert closes, Simba, along with Timon and Pumbaa and the Lion Guard, show up to pay their respects. Simba suggests that his family joins him in singing a special song from his past and Ma Tembo and the elephants happily agree.
Ma Tembo is seen heading towards Pride Rock for the Savannah Summit with several other leaders, including Makuu. She is one of the few animals that accepts Makuu being at the event. She then heads to Mizimu Grove, eventually getting comfortable and snuggling up to everyone during the song, Welcome to the Summit.
In an effort to find out who's plotting against Makuu, Kion convincingly tells the leaders that Makuu completed his journey through the Circle of Life, with Ma Tembo expressing sadness. When Makuu is revealed to be alive and well and forgiving towards those who wronged him, she lets out a large cheer as the summit continues.
One morning, the Lion Guard notice several animals running off in a certain direction. When Ma Tembo rushes past the Lion Guard, they try to find out what she's in a hurry for, but she tells them that she can't stop. She makes her way to a sausage tree, having heard of a marvelous show from other animals. When the performers arrive, Uroho, the leader of The Traveling Baboon Show, performs a magic trick with her, with shocks the elephant. After enjoying the show, she and most of the animals leave.
But when they discover a large patch of land devoid of food, she calls for the Lion Guard to help assist her. With the food disappearing so suddenly, she is certain that there's a food thief, and the Lion Guard agree to help. Ma Tembo then guides the other animals away to find more food, leaving the Guard to their work. A bit later, after being discovered as the thieves, Uroho offers Ma Tembo a fruit which she graciously accepts. Feeling bad, the baboons offer her and the other animals a free show, as their way of apology.
When Janja's Clan attacks her herd, Kion and the Lion Guard rushes to protect them. Ma Tembo remains calm during the ordeal, instructing her herd to protect the younger members of the herd.
After being rescued, Kion questions upon their wellness, and Ma Tembo warmly assures and thanks him, Fuli comments upon her disbelief of the hyenas attacking the elephants, and Ma Tembo sympathetically replies that food must be scarce in the Outlands, to which Kion responds by stating the better chances they'd have if they respected the Circle of Life.
Ma Tembo agrees, but reminds Kion of the hardships of the Dry Season. After Ono questions her, Ma Tembo denies having found a water source, but explains that she'd never led a search before, and it was harder then expected to hear underground water, even without hyena attacks. Beshte states her safety to continue searching now the hyenas had gone, and her herd agrees. Ma Tembo agrees, and embraces Kion, confessing that the responsibility of caring for others during the Dry Season was more than she expected. Kion sympathizes with her, and upon their departure, sends Ono to keep an eye on them.
Later, the hyenas attack again, targeting the baboons that have begun to follow the elephants, and the Guard arrives to defend them. Ono and Ma Tembo lead the elephants to the base of a cliff, with the baboons following. Once assembled, Kion confronts Janja, orders him to leave, and barely restraining his anger, unleashes the Roar. When the hyenas have gone, Kion questions Big Babon, and he states to have been following Ma Tembo. Ono and Ma Tembo explain the reason, and after Kion questions her, Ma Tembo states her responsibility, and role in the Circle of Life. She goes on to state that things will be well once the new water source is found. Bunga chimes in with his assurances, and Ma Tembo warmly agrees, remarking her near preference of the hyenas to baboons, a statement that is echoed by Fuli. Kion wishes Ma Tembo luck, and Ma Tembo thanks him.
As the Guard leaves, with Beshte and Kion expressing their concerns and regrets at not being able to provide additional assistance, the elephants and baboons begin chanting, which quietly overwhelms her. Observing the scene, Kion decides to share the trouble with his father.
Later, Kiara asks her parents for permission to help Ma Tembo, and is allowed to do what she can. Accompanied by Makini, she departs. They arrive to find Ma Tembo surrounded by her herd, and several impatient Pride Landers. Kiara questions on what could be done to help, and Ma Tembo states not knowing if anything could be done, believing to be in the general area, but having not found the well site. Makini suggests looking for signs, and Ma Tembo agrees, praising Makini, and thanking Kiara. Kiara states her gladness to help, and leads Makini away.
Later, still surrounded by animals, Ma Tembo attempts to locate a water source, but is distracted by the chattering of the animals. Kion questions her, and Ma Tembo denies having had luck, stating that the chattering around her was making it harder. Makini realizes what a earlier lesson from Rafiki meant, and shushes the animals, giving Ma Tembo the peace and quiet she needed.
Ma Tembo locates the new source, and leads the animals. Upon arrival, she instructs her herd to dig, and the Lion Guard joins in to help. With the new water source unearthed, Ma Tembo gladly announces the discovery. Kion warmly praises Ma Tembo, and they agree that responsibility means never giving up, despite the frustrations.