Ma Tembo is seen at her father's funeral with the rest of her herd. She watches the other elephants lay their tributes, and smiles when a baby elephant lays their flowers down upon the corpse of her late father.
Later, she announces that Simba will be saying a few words about her father. She listens to Simba's mistake in speaking elephantese, and, although at first appears surprised and then furious, she simply laughs. She admits that her father did indeed have 'poop' on him, and then thanks Simba, whilst recalling amusing times with her father.
Ma Tembo hosts The Elephants' Concert, although Simba is unable to attend after falling down a sink hole. She is seen conducting the concert, with Kiara and Nala attending instead. She is honored to have two members of the royal family attend, and she shows her respect by bowing to them. Shortly after the concert closes, Simba, along with Timon and Pumbaa and the Lion Guard, show up to pay their respects. Simba suggests that his family joins him in singing a special song from his past and Ma Tembo and the elephants happily agree.
Ma Tembo is seen heading towards Pride Rock for the Savannah Summit with several other leaders, including Makuu. She is one of the few animals that accepts Makuu being at the event. She then heads to Mizimu Grove, eventually getting comfortable and snuggling up to everyone during the song, Everyone is Welcome.
In an effort to find out who's plotting against Makuu, Kion convincingly tells the leaders that Makuu completed his journey through the Circle of Life, with Ma Tembo expressing sadness. When Makuu is revealed to be alive and well and forgiving towards those who wronged him, she lets out a large cheer as the summit continues.