Kiburi is a long, sleek reptile with large, sharp teeth and patterns emerging from under his tail along with two dark green spots under his chin. A dark green rims his eyes, and is also present on the back of his head. He has three green markings on the side of his ribs. His underbelly is light green. Running along the end of his tail are green stripes.
Kiburi is an arrogant and egotistical crocodile who believes that violence is the solution to every problem and who dislikes the notions of sharing and compromises, much like Makuu did before becoming a responsible leader. Kiburi is also very disrespectful and ill-mannered of Makuu's authority, just like Makuu did not respect the authority of the previous crocodile leader, Pua. Unlike Makuu, Kiburi is shown to be foolhardy and overconfident to the point where he fails to think things through, as shown when he challenges Makuu to a Mashindano and tries to assassinate Simba, only to have both backfire and end up not only banished from the float by Makuu, but the Pride Lands by Simba. He also appreciates the intellect of others like Ushari. Kiburi seems to have resentment for Simba, refusing to listen to him and is quick to turn to assassinating him when the notion is suggested to him. He is also far more power-hungry than Makuu, who simply wanted to be leader of the float and tried to take over only the watery areas of the Pride Lands before he reformed.
While trying to stop a herd of Sable Antelopes, Ono guides them into a dead end canyon, where Makuu's Float are resting. The stampede causes the cave to tremble, and wakes up the entire float. Upon learning that Ono is the reason of why they're now awake, Kiburi immediately threatens to eat him but Makuu stops him. Although Makuu is willing to listen to the Lion Guard's suggestions, Kiburi is not so willing. After warning Kiburi, Makuu leaves to speak with King Simba and orders his float to stay put until his return. But Kiburi refuses to do so, and decides that the crocodile way is to take the water they need regardless, and the crocodiles follow him out.
After scaring some animals away from their water source, Kion tracks down Kiburi and the two get into a brief fight until Makuu arrives with Simba and angrily orders Kiburi to stop. The Lion Guard proceed to direct them to a new watering hole. But the watering hole is cramped and uncomfortable and Kiburi demands more. Whilst Makuu is still grateful for the compromise, Kiburi challenges him to a Mashindano for leadership, to which Makuu agrees.
Ushari, having heard about the events from Shupavu who has been spying on the crocs, reports this to Scar, who orders him to trick Kiburi just before the Mashindano, into thinking that if he can take down Simba, it will leave the Pride Lands to the crocodiles' leadership. Gathering up three other crocodiles, Kiburi agrees, and sends Tamka and two others to attack Simba while he fight Makuu in the Mashindano. Despite putting up a good fight, Kiburi is ultimately defeated by Makuu. Upon noticing that Simba has vanished, he proudly announces that his crocodiles took down King Simba during the Mashindano, and that the Pride Lands now belongs to the crocodiles. But when Simba arrives, he is fully aware that Kiburi was behind the plot, which the Lion Guard foiled. When Makuu passes punishment over to Simba, he approaches Kiburi and announces that he and his followers are now exiled from the Pride Lands. Kiburi tries to protest but is denied any reasoning, and leaves the Pride Lands, promising that they haven't heard the last of him.
In the Outlands, Kiburi finds Ushari coiled up asleep. He angrily confronts him. Ushari is relaxed and, when Kiburi asks why he shouldn't eat him, the cobra calmly persuades the crocodile to let him show him something very, very special. He leads the crocodiles to the Outlands Volcano, where Kiburi is at first unimpressed until Scar reveals himself. After assuring Kiburi that they're all friends in the Outlands, he starts to explain how he has a plan, to gather all the Outlanders and take over the Pride Lands again.