Jasiri was the first member of the clan to be seen. When Kion lost his way into the Outlands, he came across the female hyena. While initially distrustful of her, they learned to trust one another and Kion learned that not all hyenas are bad like Janja and his clan and they have more in common then he suspected. Jasiri guided him through the Outlands back home, but was attacked by Janja and his clan. Kion joined the fight and managed to drive them off by using the Roar of the Elders. The two then separated with Kion returning with the rest of the Lion Guard to the Pride Lands and her going back into the Outlands, presumably back to her clan's territory.
Jasiri made her way into the Pride Lands in order to ask Kion for help, as a pride of strange lions living in the Outlands had taken over her clan's watering hole, and refused to share it. Kion, thinking this was just a misunderstanding, followed her into the Outlands to Jasiri's territory, much to the surprise of her sister Madoa, whom had her doubts about Kion and lions in general. The cubs of the clan, Wema and Tunu, are also afraid of lions, as they only know the pride that took over their watering hole. Kion assures them that he can convince the lions to give them their watering hole back and that they likely are unaware that good hyenas exist and thus it is just a misunderstanding.
Later, when Kion drives away Zira and the Outsiders, Jasiri's Clan are free to return to their watering hole. Madoa thanks Kion and reveals that she is now glad that Jasiri has a friend like him, and Jasiri comments that she's glad to have the entire Lion Guard as her friends. Although despite Kion's geniality, the pups are still shown to be afraid of Kion, which Jasiri comments, saying Kion should take it as a compliment for his ferocity.
When looking for the jackals Janja, Cheezi and Chungu come across Jasiri who informs the trio that they forgot to remark their turf, and that it's part of her territory now. She allows them to pass through, but a fight breaks and Jasiri manages to drive them off.
A little later, she is seen playing with her Madoa along with the cubs Wema and Tunu in a game of hide and seek until she and the cubs get attacked by Janja's Clan. Jasiri does her best to defends the cubs but the trio get knocked into a lava geyser. Madoa who, unbeknownst to Janja, has seen everything that happened and runs off to seek the Lion Guard's assistance.
She makes it to Pride Lands to tell them that her sister and the cubs are in danger. The team heads off but it is difficult with some Tsetse Flies blocking their path, so they ask Thruston to come with them. Once they make the Outlands, they find where Jasiri and the cubs are held and everyone starts to distract the hyenas, while Kion devises a plan. Beshte is able to push a stone slab into the lava geyser which allows the three to get out. Seeing that his plan has failed, Janja is the last one to retreat with his clan back, deeper into the Outlands. When everyone is safe Jasiri admits that she is a little concerned over Janja's attack, seeing it as something more than a fight for territory, but Kion consults her saying that the heat of the dry season make some animals do crazy things. She and the pups thank their friends for rescue and return home.
Jasiri is the first member of the clan to be introduced, and seems to have a certain amount of respect and authority in the clan, though its unknown if she is the actual matriarch. She is a fairly small and slim spotted hyena, though she displays strength when fighting Janja's clan. She has gray fur that bears a slight purplish tint. Unlike the hyenas Kion had previously met, Jasiri respects the Circle of Life and partakes in it by scavenging what other predators such as lions leave behind. She is very spunky and lively, and has been described by her voice actor as zany and opinionated. She is shown to be a bit teasing and sassy, not to mention confident in her fighting skills.
Madoa is Jasiri's sister. Her color scheme is largely the same Jasiri, though her spots slightly resemble stripes. She is skeptical about Kion and lions in general, but isn't afraid to admit that, while she had her doubts, Jasiri's trust in Kion was not misplaced. She is also shown to be very nurturing, as she is shown to be protective and concerned about the cubs of the clan.
Wema is one of the cubs in Jasiri's clan. She is a small hyena cub with purple fur and dark purple ears. Her legs and paws are dark purple and her tail is dark purple on top and light purple underneath. Her eyes are navy blue. Her underbelly and inner ears are a light purple. Wema is a very shy hyena cub who is afraid of lions, both Outsiders and Pride Landers like Kion. She is shown to be very playful when interacting with her brother Tunu.
Tunu is one of the cubs in Jasiri's clan. He is a small hyena cub, with grey fur and dark grey markings on his back and forehead. His paws and muzzle are also this color. His nose is black and his eyes are blue. His tail is dark grey with a light pink fur underneath. This light pink color is also present on his underbelly. Like his sister, Tunu is a very shy hyena cub who is afraid of all lions from both the Outlands and Pride Lands, even after Kion exerts warmth towards them. He is shown to be very playful, often following his sister everywhere.
This is the only group of Outlanders so far that isn't hostile towards the Pride Landers. All other Outlander groups (Reirei's Pack, Mzingo's Committee, Janja's Clan, and the Outsiders) tried at some point to invade the Pride Lands, hunt there or attack a Pride Lander.